The effect of various additives on the microbiota of ruminant rumen and the course of metabolic processes in the rumen <i>in vitro</i> studies

Relevance . In an in vitro study, changes the taxonomic scar and course of fermentation processes response to addition various lipid additives ruminant diet were studied. Methods Three samples formed: control — without oil, experimental ones supplemented with sunflower oil (3%) (I) soy (II). The object study is content obtained from Kazakh white-headed bulls chronic fistula (n = 3) by Latin square method. Studies digestibility SV carried out using incubator «ANKOM DaisyII» according a specialized technique. Laboratory studies: level volatile fatty acids (VFA) contents was determined gas chromatography on chromatograph «Crystallux-4000M», determination nitrogen forms GOST 26180-84. Microbial biodiversity rumen MiSeq («Illumina», USA) new generation sequencing method (NGS) set reagents MiSeq® Reagent Kit v3 (600 cycle). Results oils had stimulating effect feed rumen, increasing this indicator 2.7%. Compared control, reduced concentration total protein content, while non-protein increased (by 5.4%) when 7.8%) soybean oil. additional inclusion showed increase acetic butyric acids, respectively: group 46.8% 55.6%, 51.5% 60.5% relative control. change number microorganisms RYE, but qualitative composition microbiota did not significantly.